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Technical annual report 2000-01



Technical annual report 2000-01


Dept. of Regional Development, Primary Industry, Fisheries and Resources technical annual report; Department of Regional Development, Primary Industry, Fisheries and Resources technical and annual report; Reports; PublicationNT; Technical bulletin (Northern Territory. Dept. of Primary Industry and Fisheries) ; no. 295




Made available via the Publications (Legal Deposit) Act 2004 (NT).






Agriculture -- Northern Territory -- Periodicals; Fisheries -- Northern Territory -- Periodicals

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Dept. of Primary Industry and Fisheries

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Technical bulletin (Northern Territory. Dept. of Primary Industry and Fisheries) ; no. 295



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Technical Annual Report 2000/01 199 beans were obtained from Sunland Seeds, a bean wholesaler, to include in the screening program. A dwarf line was obtained from the Darwin rural area. Malcolm Smith put us in contact with Dr. P. Umaharan, University of the West Indies, Trinidad-Tobago. After e-mail communication with Dr. Umaharan, six lines of snake beans (bodi) including one line with a high level of resistance in trials in Trinidad were imported and grown in Post Entry Quarantine (PEQ) at Berrimah. Seed will be released from plants inspected as healthy in PEQ. Plants grown from this released seed will be screened in the glasshouse for resistance to Fot. This will be reported in the Technical Annual Report for 2001-2002. Four lines of snake beans assessed as having good pod characteristics were screened for resistance to the Fusarium wilt. All four lines tested susceptible. Seed from a line imported from Dr. Yi-Sheng Lin, National Chungshing University, Taichung, Taiwan called White Skin was released from PEQ. The White Skin line was reported to have a high level of resistance in tests against the Fusarium wilt in Taiwan. In the first Fusarium resistance screening in 2001, the White Skin line was found to have some resistance, but was highly tolerant to infection with a 1999 isolate of Fot (24946). The tolerance of the White Skin line was demonstrated when the Fusarium was isolated from 5/10 of the symptomless plants. Another line tested at the same time, Red Eye, obtained by Mark Traynor from a seed company, was found to be more susceptible than the standard commercial variety. To conserve space, and so thereby screen the snake bean lines more rapidly, a composite screening of ten seedlings of each line in 6-inch pots rather than screening ten seedlings in individual small pots was devised. Each seedling was tagged with a price tag for recording purposes. Since an initial trial of a few lines indicated that this method was successful, it was adopted as the standard screening technique. Inoculations of the first two screenings of composite pots of snake bean lines of 26 and 28 lines respectively were commenced on 29/5/01 and 29/06/01 by inoculating them with a 1999 isolate of Fot (24946). Both tests included positive controls. Results are expected from these two screenings early in the next financial year. Samples of wilted snake beans from a property in the Webb Road Humpty Doo area of Darwin in September 2000 yielded a Fusarium oxysporum, which was yellow-orange in colour in contrast to the plum red colour of all the Fot isolates in 1999. This yellow-orange-pale pink Fusarium was also isolated from some wilted snake beans collected in early 2001. We have not examined the relationship between these two types of isolates yet, nor have we done a survey to indicate the distribution of the two apparently different types of Fusarium. Contacts were made with scientists in Queensland, Taiwan, Mississippi State University, South Carolina, USDA at Georgia, UC-Davis, UC-Riverside and Trinidad on snake bean and cowpea Fusarium wilt disease and management issues. Efforts to identify the cowpea differential lines used by previous researchers to enable us to determine the race of the Fot isolates were unsuccessful. An updated information paper on the snake bean Fusarium wilt was prepared and distributed as extension material for farmers. PROJECT: Management Systems for Diseases of Asian vegetables Fusarium Wilt of Basil Project Officers: B. Cond and I. Arao-Arao Location: Darwin Objective: To develop a management system for diseases of basil with present emphasis on Fusarium wilt and base rot. Background: Fusarium wilt of basil (Fusarium oxysporum f.sp. basilici) was identified in the Darwin rural area in 1997, probably introduced in infected seed some three years earlier. It again became a problem in May 2000 when Vietnamese farmers began to grow basil and submitted affected plant samples to Plant Pathology with typical external and internal symptoms.