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The effect of true water hardness and alkalinity on the toxicity of Cu and U to two tropical Australian freshwater organisms



The effect of true water hardness and alkalinity on the toxicity of Cu and U to two tropical Australian freshwater organisms


Riethmuller, N.


E-Publications; E-Books; PublicationNT; Internal Report 348




Made available via the Publications (Legal Deposit) Act 2004 (NT).






Internal Report 348

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Two plastic trays, one of such a size to hold sixteen 50 mL beakers and the other to hold twenty-four 50 mL beakers Pasteur pipettes, with internal tip diameter of - 2 mm 8.1.8 Test environment The preparation and storage of test solutions, culturing of test hydra, and conducting tests should be can'jed out in premises free from harmful vapour, dust, and any undue disturbance. All workers involved in any part of the test should wash hands and arms thoroughly with fragrance free soap and rinse well with tap water before commencing any part of the test procedure. 8.1.9 Data recording Test animals are observed and data recorded at 24 h intervals after the commencement of the test (when t = 0 h). Observations made at the end of the first 24 h period are designated as Day I observations; at the end of the second 24 h period, Day 2. observations etc. Water parameters are measured and adjusted (where appropriate) and recorded at the beginning and end of each 24 h period, and are designated as Fresh Water Day 1, 24 h -old Water Day 1, re:::;pectively, and so forth during the test. 8.1.10 Test procedure Day 1 1. Prepare the te:::;t solutions (as outlined in Section B.1.6) and leave at room temperature. 2. I:::;olate approximately 250 suitable hydra in synthetic water in a Petri dish and leave at room temperature. A 'suitable test hydra' is a hydra with one bud. The bud must not be fully developed (ie. tentacles are present only as 'bumps', and the bud must not appear ready to detach from the main stem of the hydroid). 3. Dispemie 30 mL aliquots of each test concentration (normally 8) into 3 appropriately labelled replicate Petri dishes (ie. 3 x 30 mL for each test solucion), and arrange in three replicate groups on clear plastic trays (eg. Control replicate I to X flg LI 011 Tray I). .:I.. Using a microscope and Pasteur pipette. pick out one hydra from the isolated stock and place into Control repliclte I. 5. Repeat for remaining test concentrations of replicate I, working up in concentration. ;.100 ending with the highest concemratiun. 6. Discard the Llsed pipette ;.tnd select a new one. 7 Repeat ~ter" -L-h until all te"t di"hes fe\!' rhat !'epli(:clte group c{:ntain ! 0 hydra. 104

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