Portable electronic nose technology to detect pathogens and diseases in plants
Details:
Title
Portable electronic nose technology to detect pathogens and diseases in plants,
Creator
Northern Territory. Department of Industry, Tourism and Trade. Plant Biosecurity Branch,
Collection
E-Publications,
E-Books,
PublicationNT,
Date
2023-12-20,
Notes
Made available by via Publications (Legal Deposit) Act 2004 (NT),
Table of contents
Acronyms -- Acknowledgement --Executive Summary -- Introduction -- What is an E-nose device? -- E-nose devices -- Cyranose 320 -- PEN 3 – Portable Electronic Nose 3 -- MSEM 160 – Multi Sensor Environmental Monitor 160 -- Sampling / analytical techniques -- PCA – Principal Component Analysis -- LDA – Linear Discriminant Analysis -- CD Analysis – Chemo metric Data Analysis -- Method of Cross Validation -- Plant Pathogen applications used to study e-Nose device capability -- Tropical Race 4/Panama Disease (Fusarium Wilt) -- Mango Twig Tip Dieback (MTTD) -- Objectives -- Methods -- Experiment 1: Assessing unit functions and E-nose sampling on known volatiles -- E-nose sampling on mixture of volatiles -- Experiment 2: Volatiles from pure cultures of Plant pathogens and controls for training -- Experiment 3: Laboratory based trials -- GC-MS analysis - Data acquisition -- Data Analysis -- E-Nose sampling -- Sampling of healthy and infected plant substrates -- Experiment 4: In field trials (or mock real-time trial under laboratory conditions) -- Healthy and Mango Twig Tip Dieback infected mango leaves – Field collection -- Healthy and Tropical Race 4 infected banana pseudo stems- Field collection -- Results and Discussion -- Experiment 1: Assessing unit functions and E-nose sampling on known volatiles -- Cyranose 320 – Basic device -- PEN 3 -- MSEM 160 -- Experiment 3: Laboratory based trials -- Tropical Race 4 cultures in Potato Dextrose Agar media and uninoculated Potato Dextrose Agar as the control -- E-nose Sampling Results -- Mango Twig Tip Dieback (Pantoea sp. and Staphylococcus sp.) cultures in Nutrient Agar media and uninoculated Nutrient Agar as the control -- MS Results -- E-nose sampling -- Healthy and MTTD infected mango leaves – in vitro -- Experiment 4: In field trials (or mock real-time trial under laboratory conditions) -- Healthy and Dieback infected mango leaves – Field collection -- Healthy and Tropical Race 4 infected banana pseudo stem samples – Field collection -- Comparative analysis -- Conclusions -- Future directions and applications -- Other applications for e-nose device -- Applications for each unit -- Future funding opportunities / sources of investment -- References -- Appendices,